SAL1 is localized to the plasma membrane. Subcellular localization of WT or mutated SAL1-GFP under the CaMV 35S promoter control. Each construct indicated in the figure was transformed into rice protoplasts for GFP and chlorophyll fluorescence observation. Single, double, or triple Cys-to-Gly mutations of three potential sites Cys12, Cys151, and Cys152 were generated to examine their effects on the SAL1 subcellular localization. Scale bar = 5 μm.