Temsirolimus and recombinant AMH decreased cyclophosphamide-induced DNA damage and apoptotic oocyte death. Ovary sections from (A) representative hematoxylin and eosin images of ovaries from control (Con, left) and Cyclophosphamide (Cy, right) treatment groups demonstrating positive (red arrows) and negative (yellow arrows) phosphorylated form of histone 2AX (γH2AX) staining. (B) Ovaries removed 12h, or (C) 24 h after Cy (also C) treatment, were stained for DNA damage using γH2AX antibody, and the apoptotic marker cleaved poly (ADP-ribose) polymerase (cPARP) antibody. Primordial follicle oocytes from one or two sections from six to eight ovaries per group were evaluated (at least 70 follicles from each group), follicles were classified as positive/negative. *P < 0.05, **P < 0.01, and ***P < 0.001. Data were expressed as the mean ± SEM. Statistical analyses were performed by the mixed procedure followed by Tukey’s multiple comparison test. Tem and T: Temsirolimus; rAMH and A: recombinant anti-Müllerian hormone.