Temsirolimus and recombinant AMH in vivo primordial follicle counting. Ovaries from 8-week-old mice that were removed 21 days after a single dose of Cyclophosphamide (Cy, 150 mg/kg) or the equivalent volume of PBS with or without cotreatment with Temsirolimus (Tem) and/or recombinant anti-Müllerian hormone (AMH) and were stained with hematoxylin and eosin for follicle count: primordial follicles were counted 21 days after Cy treatment. Fourteen to 16 ovaries were counted for follicles per group, ***P < 0.001. Data were expressed as the mean ± SEM. Statistical analyses were performed by the mixed procedure followed by Tukey’s multiple comparison test.