Role of N- and C-terminal regions in polar localization of OsLsi1. A) Scheme for OsLsi1 structure and deletion region of OsLsi1. The deletion regions at the N- and C-termini are indicated in orange lines. TMD indicates the transmembrane domain. B to E) Localization of OsLsi1 variants in roots. Localization of Flag-OsLsi1 B), Flag-OsLsi1^d2–40C), Flag-OsLsi1^d265–298D), and Flag-OsLsi1^{d2–40, d265–298}E) in roots of transgenic lines. Cross-sections of the mature region (15 to 20 mm from the root tip) of the crown roots were used for immunostaining with an antibody against Flag-tag. Exodermis (Ex) and endodermis (En) were enlarged in the middle or lower panel, respectively B) to E). Red color indicates the signal of immunostained Flag-tagged proteins. Scale bars indicate 50 μm in the whole root picture or 10 μm in the enlarged picture B) to E).