CDR3βAA of untreated NOD mice have higher convergent recombination (CR) levels than clones from transplanted NOD mice. CDR3βAAs (complementary-determining region three) associated with T1DM-antigens are enriched in the high CR clones from NOD mice. (A) CR vs. frequency of CDR3βAA from transplanted NOD mice—with veto cells (CHV), from untreated NOD diabetic mice (NOD-T1DM), or healthy untreated pre-diabetic NOD mice (NOD-pre T1DM). Each dot represents a single β chain CDR3AA sequence. Annotated-T1DM-related clones with high CR (CR > 4) are indicated. (B) CDR3βAA sequences in transplanted NOD mice have significantly lower CR levels than untreated NOD mice (Wilcox test). Each bar represents the mean CR levels of each mouse group. Error bars indicate SEM (n ≥ 3). (C) Table of annotated-T1DM-related CDR3βAA sequences with CR > 5; these sequences are significantly more frequent in untreated NOD vs. the transplanted NOD mice (CHV) (P < .05, Wilcox test).