Some cell wall components differ among developmental stages. (A–X) Representative images of immunofluorescence with antibodies (A–F) CBM3a, (G–L) LM11, (M–R) LM19, and (S–X) LM20. (A–C, G–I, M–O, S–U) Autofluorescence. (D–F, J–L, P–R, V–X) Antibody signals. Different sections from the same samples were used for different antibody labeling. The AZ and its surrounding tissues are manually divided into AZ-stalk, AZ-seed, photosynthetic ovary wall on the palea side, and the lemma side. Images are enhanced for visualization, and raw images were used for quantification. Scale bars = 50 µm. (Y) Signal intensity of the 3 biological replicates (circles) and mean ± standard error (lines) within each region for each antibody. Stars denote significant differences (Tukey's test, P < 0.01) between developmental stages. S2, seed filling stage; s3-attached, early seed maturity stage before abscission; s3-detached, post-abscission stage. g, green tissue at the bottom of the caryopsis; ts: triangular stalk.