Trypan blue staining shows cell death in the caryopsis AZ of E. tef. (A) Examples of E. tef spikelets stained with Trypan blue. Scale bar = 1 mm. (B, C) Dissected florets indicated by arrows on the left and the right from (A), respectively. Scale bars = 500 µm. (D, E) Enlarged images of the triangle stalks from (B) and (C), respectively. Scale bars = 50 µm.