Application of the LRLoop to retinal development. (A) Five major cell types identified using scRNA-seq dataset during the early retinal developmental stages (E14 and E16). (B) Sc score between the five cell types calculated using LRLoop during early stages. (C) The unique ligand–receptor interactions predicted between RGCs and neurogenic cells during early stages. The red arrow indicates the known interactions. (D) Detailed feedback loops for interactions between RGCs and neurogenic cells during early stages of retinal neurogenesis. (E) Sc score between the six cell types calculated by LRLoop during intermediate stages. (F) Sc score between the six cell types calculated by LRLoop during late stages