LRLoop algorithm and its assessment. (A) Schematic of the LRLoop concept. Ligand 1 (L1) interacts with receptor 1 (R1), while ligand 2 (L2) interacts with receptor 2 (R2). Furthermore, L1 is the downstream target of R2 through signaling pathways and gene regulatory networks (GRNs). Similarly, L2 is the downstream target of R1. (B) The flowchart of LRLoop. (C) An example feedback loop between Muller glia and microglia