Fig. 7.
Wet Lab module within the metadata schema. Full schema available in Supplementary Figure S1. The Wet Lab module is related to the Field Survey module through the relationship between the Extraction and Field Sample tables. Extractions and pooled libraries are uniquely identified through the Sample Barcode table. qPCR and ddPCR metadata were documented in the PCR and PCR Replicate tables and through the relationship of the PCR table to the Primer Pair table. The location of wet lab preparation is documented through a table’s relationship to the Process Location Table. Associated standard operating procedures are also documented through the relationship to the Standard Operating Procedure table. All other metadata collected throughout the library preparation and sequencing steps are documented in the NGS-specific tables: Library Prep, Index Removal Method, Quantification Method, Amplification Method, Size Selection Method, and Index Pair. Library preparation primers are selected through the Primer Pair table, and proceeding NGS tables include Pooled Library, Run Prep, Run Result, and FASTQ File. Full descriptions of fields for the Wet Lab module are available in Supplementary Table S4

Wet Lab module within the metadata schema. Full schema available in Supplementary Figure S1. The Wet Lab module is related to the Field Survey module through the relationship between the Extraction and Field Sample tables. Extractions and pooled libraries are uniquely identified through the Sample Barcode table. qPCR and ddPCR metadata were documented in the PCR and PCR Replicate tables and through the relationship of the PCR table to the Primer Pair table. The location of wet lab preparation is documented through a table’s relationship to the Process Location Table. Associated standard operating procedures are also documented through the relationship to the Standard Operating Procedure table. All other metadata collected throughout the library preparation and sequencing steps are documented in the NGS-specific tables: Library Prep, Index Removal Method, Quantification Method, Amplification Method, Size Selection Method, and Index Pair. Library preparation primers are selected through the Primer Pair table, and proceeding NGS tables include Pooled Library, Run Prep, Run Result, and FASTQ File. Full descriptions of fields for the Wet Lab module are available in Supplementary Table S4

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