Mitochondrial morphology in slo3. A: Representative images of the protoplasts. From left to right: mitoTracker stain for mitochondria, SYBR Green I stain for nucleic acids, especially for mitochondrial nucleoids, merged image of two fluorescent images, and DIC (differential interference contrast). Scale bars = 10 µm. B–E, Protoplast analysis; number of mitochondrial particles per cell (B), mitochondrial area per cell (C), number of mitochondrial nucleoid bodies per cell (D), and percentage of mitochondrial particles with nucleoid (E). A total of 829 mitochondrial particles from 22 cells in the wild type and 1,601 mitochondrial particles from 19 cells in slo3 were analyzed. Characteristics of the boxplots are similar to Figure 1G. * indicates statistical significance by Student's t test (P < 0.05).