Seed oil content in gene-silenced and loss-of-function mutant lines. A, Relative expression of BnaCIPK9 in developing seeds from T0 to T2 generations. The independent transgenic plant lines with Napin and 35S promoters, respectively. Each independent line included 3–4 inbred lines as biological repeats, and each inbred line had three technical repeats. Error bars indicate standard deviation (n = 9–12). B, Seed oil content in RNAi line mature seeds. Both the T1 and T2 generations were grown under field conditions. C, Seed oil content in bnacipk9 lines. The T2 and T3 generations were grown under growth chamber and field conditions, respectively. B and C, Each independent line included 5–7 inbred lines as the biological repeats. Error bars indicate the standard deviation (n = 5–7). The seed oil content was measured using the NIRS. DSW, dry seed weight. Each dot denotes one plant of the mixed inbred lines within each independent line. Single, double, and triple asterisks indicate significant differences between the transgenic and WT plants with P values of 0.05, 0.01, and <0.001, respectively, as determined using the analysis of variance by the least-significant difference test.