CO₂ assimilation rate as a function of intercellular CO₂ partial pressure at different temperatures in wild-type (WT) S. viridis and transgenic plants overexpressing SBPase. Measurements were made on the T₂ progeny of lines 3 and 5 at an irradiance of 1,500 µmol m⁻² s⁻¹ and a leaf temperature of 35°C or 15°C. Average relative abundance of SBPase per leaf area, calculated from the immunoblots in Supplemental Figure S1, was increased two times in line 3 and 1.5 times in line 5, relative to the WT. Mean ± Se, n = 3 biological replicates. Details of statistical analysis of CO₂ assimilation rates between transgenic and WT plants are provided in the results (P < 0.05, t test).