(A) As in wild type, HO induction increased likelihood of silencing loss in a swi2∆10R mutant (JRY10820). Early silencing-loss events were defined as above. As a negative control, part of the culture was maintained in raffinose. A minimum of 460 colonies was analyzed for each time point. For all time points other than 3 hr in galactose and 4 hr in galactose, the means and SD of three independent biological replicates are shown. For the 3- and 4-hr galactose time points, the means of two independent biological replicates are shown. (B) A DNA hybridization blot evaluating HML::cre and pseudo-MAT showed double-strand break induction at pseudo-MAT after HO induction in swi2∆10R (JRY10820) for the times shown. (C–E) Gene conversion frequencies in swi2∆10R at pseudo-MAT after 1-hr HO induction in (C) all colonies, (D) colonies that experienced an early silencing-loss event, and (E) colonies that maintained silencing. For each of the three SNPs distinguishing pseudo-MAT and HML::cre, the percentage of colonies that showed evidence of gene conversion is shown. Gene conversion events were scored as in previous figures. The means and SD of three independent biological replicates are shown. For data points with overlapping error bars, the error bar color matches its reference data. A minimum of 125 colonies was analyzed for each replicate of the overall population shown in (A).