Deletion of RPD3 increased Sir4 association with the silencers. Sir4-myc ChIP was performed in the MATa  sas2Δ hmra1Δ::URA3 with the following SIR2 and RPD3 genotypes: SIR2 RPD3 (JRY10635), sir2N345A RPD3 (JRY10632), and sir2N345A rpd3Δ (JRY10633). No enrichment was observed in a strain not expressing the Sir4-myc (JRY10634). Enrichment is shown as the Immunoprecipitation (IP)/input values relative to the SEN1 IP/input values as a negative control. Error bars represent standard error of four biological replicates. The asterisks indicate a P-value of <0.05 (Student’s t-test).