Approach for flow cytometric analysis. (A) Fluorescence minus one (FMO) controls of the used panel of markers (CD16, CD11b, CD62L, CD10). CD64 was also tested in this panel but is not shown here because it is not discussed further in this article; (B) gating strategy for analyzing the blood samples from healthy controls and patients. Flow cytometric analysis was done using FlowJo analysis software (Tree Star Inc.)