(A) Delayed type hypersensitivity test (DTH). One week after the completion of the immunization program, the mice were inoculated in the plantar pad with 50 µl of OVA (0.4 mg/ml). The thickness of the plantar pad was measured at the beginning of the experience as well as 24 and 48 h after inoculation. The bars represent the results of the differences between both measures (ΔA) ± SD and are representative of 2 independent experiments performed. *P < 0.05 and ***P < 0.001 compared with OVA; +++P < 0.001compared with Al(OH)3 at 24 h; ###P < 0.001 and ##P < 0.01 compared with Al(OH)3 at 24 and 48 h, respectively. (B) Representative photographs of plantar pads of the hind legs of mice from the evaluated groups by DHT test at 48 h post OVA injection. The left (L) hind legs were used to challenge with 0.05 ml of OVA (0.4 mg/ml) and the right (R) hind legs were used to inoculate sterile saline solution and served as a control. (a) Intradermal inoculation in the plantar pad, (b) pad thickness measurement with caliber, (c) OVA group, (d) OVA + Al(OH)3 group, (e) OVA + EO group, (f) OVA + Al(OH)3 + EO group
