Knockout (KO) of IL-17a attenuated hepatic ischemia–reperfusion (IR) injury in mouse model with fatty liver. (A) The serum IL-17a levels in IL-17 WT mice with fatty liver and normal livers were examined by ELISA at 6 h after hepatic IR injury. (B) Representative H&E stained liver sections from liver tissues of IL-17 WT and KO mice with fatty liver and normal liver at 6 h after hepatic IR injury. Red arrows pointed to the hepatocellular necrosis areas. Bar = 200 μm. (C) To compare the liver function between IL-17a KO and WT mice in response to IR injury, serum ALT level at 6 h after hepatic IR injury was detected using the mouse ALT kit. (D) Hepatic TLR4, IL-1β, IL-6, CXCL1, and CXCL2 mRNA expressions at 6 h after IR injury in IL-17 KO and WT mice with fatty liver were detected by RT-PCR. (E) The neutrophils were stained by anti-mouse Ly-6G antibody in the groups of IL-17a KO and WT mice with or without fatty liver after hepatic IR injury. Bar = 100 μm. The bar chart represented the numbers of positive Ly-6G cells per field in each group. ^#P < 0.05 between groups of fatty liver and normal liver. *P < 0.05 between groups of IL-17a KO and WT mice