Imaginal disc growth restriction during the regeneration checkpoint is the result of reduced ecdysone signaling. Ecdysone levels are rate-limiting for imaginal disc growth during the regeneration checkpoint. 20-Hydroxyecdysone (20E) rescues growth restriction induced by (A) targeted wing damage (Bx > eiger), (B) systemic dilp8 misexpression (Tub > dilp8), (C) systemic NOS misexpression (hs > NOS), and (D) PG NOS overexpression (phm > NOS) compared to control ethanol fed only larvae (EtOH). Statistical analysis: mean ± SD. *P < 0.05 and ****P < 0.001 calculated by two-tailed Student’s t-test.