Centromere location and shape are maintained across lineages. (A) High-resolution view of centromere 2 CENH3 enrichment in B73 and seven partial-B73 inbred stocks. “CENH3 fold enrichment” is the normalized ratio of CENH3 ChIP reads to total nucleosome control reads per 20-kb locus across the genome. Only uniquely mapping reads were included in this analysis. Gaps in the plot are due to exclusion of loci with <50 control reads from the analysis. The B73 used for this analysis is the Dawe lab “reference” (see Figure 2). (B) High-resolution view of centromere 2 CENH3 enrichment in purebred B73 stocks, as in A. (C) High-resolution view of centromere 5 CENH3 enrichment in B73 and seven partial-B73 inbred stocks, as in A. (D) High-resolution view of centromere 5 CENH3 enrichment in purebred B73 stocks, as in A. (E) Apparent shift in CENH3 center of mass is related to ChIP efficiency. The center of mass shifts in Table 2 were plotted against CENH3 ChIP enrichment, and linear regression lines were plotted for centromere 2 and 5 separately.