Overview of mating procedure and selection of fusants from two parent strains P1 and P2. Photographs are included to illustrate key steps, namely the area of mating on YPD plates, marked by a dashed rectangle, examples of large colonies, marked with arrows, on minimal medium plus MPA and gel images of MTL allele-based PCR products of parents and three putative fusants, PF1, PF2, and PF3. PF2 is an example of a putative fusant, which was not confirmed as a fusant by PCR (false positive).