BNP codes for a DC1-domain-containing protein. (A) Schematic view of a region of Arabidopsis chromosome 2 (from 18,323,538 to 18,321,537 bp-TAIR10) showing BNP genomic location and bnp-1 and bnp-2 insertion sites. (B) BNP amino acid sequence with boxed DC1 domains. (C) Phylogenic tree of 140 DC1-domain-containing proteins in Arabidopsis. Bootstrap test results (1,000 replicates) of the major nodes are indicated. IDs of sequences grouped in clusters I–VIII are listed. (D) Alignment of BNP with its closest Arabidopsis homologs and CaDC1 (AEI52549), TaCHP (ACU80555), NtDC1a (BAF80452) and NtDC1b (BAF80453). Phylogenic tree with bootstrap test results (1,000 replicates) for the alignment of the complete 18 sequences is shown. Amino acid region represented for each sequence is indicated. DC1 domains are framed, and signature residues and binding loop regions are indicated (C = Cys, H = His, ― = loop). Higher intensity in gray scale denotes higher sequence similarity.