Computer simulations. (A) Computer generated data with serial dilutions. Red, yellow, green, blue represent undiluted concentrations, 1/2, 1/4, 1/8 original concentrations, respectively. (B) Serial dilution plot. The blue line shows the estimated serial dilution curve. (C) The estimated versus the ‘true’ concentrations. The dashed lines show the upper (shown in green) and lower (shown in blue) bounds of the estimated concentrations according to Equations (5) and (6). The red line shows the identity lines. (D) Estimated error rates. CV=estimated error/estimated concentration. (E) Signal versus estimated concentrations. Red, yellow, green, blue represent undiluted concentrations, 1/2, 1/4, 1/8 original concentrations, respectively.