The differential scavenging capacity of ROS between the s1,2 mutant and WT plants. (A) Histochemical analysis of O₂^{• −} accumulation by NBT staining of rosette leaves from the 28-day-old s1,2 mutant and WT plants. (B) Histochemical analysis of H₂O₂ accumulation by DAP staining of rosette leaves from the 28-d-old s1,2 mutant and WT plants. (C) Growth inhibition assays of the s1,2 mutant and WT plants using 3-AT and PQ. Representative pictures of 21-day-old plants are shown. (D–E) Relative fresh weights (D) and relative Chl levels (E) of the 21-day-old s1,2 mutant and WT plants grown on medium with different levels of 3-AT or PQ. All data are means and SEs (n = 12 plants/genotype). Different letters indicate significant differences (Duncan’s multiple range test; P < 0.05).