An example illustrating the results of the TSSi analysis for 5′ tag data. The horizontal axis is the genomic position of the transcribed DNA sequence, in this example on scaffold 1. Negative numbers on this axis indicate the anti-sense strand, i.e. transcription in the plot occurs from left to right. The raw reads at positions of the respective 5′ ends of the template DNA sequences are plotted as gray dots. The measurements processed by the regularization procedure are plotted as black lines and the predicted TSSs are depicted as black circles. The gray line indicates the contamination threshold level. For this illustration, λ₁=1, λ₂=0.1, τ_5′=5 and τ_3′=10 have been chosen