Figure 2.
LacI binding sites in E. coli and chloroplasts. ( a ) Arrangement of lacO repeats in transplastomic lacO lines. lacO sequences are separated by HaeIII restriction sites, with an EcoRI restriction site at each end. The sequence of the 118-bp region is given below the diagram. The gap indicates the centre of the dyad symmetry. ( b ) Core regions of three lacO sequences from E. coli . Bases identical to lacO1 are in bold, non-identical bases are lower case. ( c ) Chloroplast DNA sequences showing similarity to lacO . Bases identical to lacO1 are in bold, non-identical bases are lower case. The locations of the sequences in the tobacco genome (Genbank Z00044) are: rrn23 , 109075-109095 and 133535-133555; ndhB , 98558-98578; ndhJ , 51302-51322; rpl14 , 83504-83524; atpBE , 57377-57397; psaA , 42101-42121; rrn16 , 103808-103828 and 138802-138822.

LacI binding sites in E. coli and chloroplasts. ( a ) Arrangement of lacO repeats in transplastomic lacO lines. lacO sequences are separated by HaeIII restriction sites, with an EcoRI restriction site at each end. The sequence of the 118-bp region is given below the diagram. The gap indicates the centre of the dyad symmetry. ( b ) Core regions of three lacO sequences from E. coli . Bases identical to lacO1 are in bold, non-identical bases are lower case. ( c ) Chloroplast DNA sequences showing similarity to lacO . Bases identical to lacO1 are in bold, non-identical bases are lower case. The locations of the sequences in the tobacco genome (Genbank Z00044) are: rrn23 , 109075-109095 and 133535-133555; ndhB , 98558-98578; ndhJ , 51302-51322; rpl14 , 83504-83524; atpBE , 57377-57397; psaA , 42101-42121; rrn16 , 103808-103828 and 138802-138822.

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