![Reduced male fertility found in different cPGI-deficient lines. A, A representative inflorescence stem showing the seed setting of the heterozygous cpgi-3(+/−) mutant, the cpgi knockout mutant expressing p35S::cPGI-YFP [p35S::cPGI-YFP cpgi-3(−/−)], the transgenic line overexpressing p35S::cPGI-YFP (cPGI-ox), the cPGI co-suppressed line (cPGI-co), the WT plant (Col-5), and the RNAi knockdown lines Ri-cpgi-1, Ri-cpgi-3, and Ri-cpgi-10. B, Alexander-stained whole-mount anthers were inspected using light microscopy for the viability of mature pollen. A representative image is shown for the plants shown in the same column in (A). Note that most of the pollen degenerated in those plants, producing mostly seedless siliques, while cpgi-3(+/−), cPGI-ox, WT, and Ricpgi-10 plants produced viable pollen and thus produced fertile siliques. Bars in (A) and (B) are 1 cm and 50 µm, respectively.](https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/plphys/191/1/10.1093_plphys_kiac494/1/kiac494f1.jpeg?Expires=1750437002&Signature=r7o2PtrBlNozv~2OlL9ORYK7ZXvRfVW5O-kpaG0sQb1hMR-NE~IGLovWQuio96OLcogLU2bqR9csITBj5xPziUDARTGJvaOk~~O5brUJ8APMBfNvMIwX0hoIMc5zf-b0Mat53dGDWCKKc6oq-ClikgVk6tPnEoptDdcdGYwR3zlAanXAkvloMG0hkeWilVSm1c3hI~NCFISm-23JnFh3-2FHi6wJEWTrxUPSbItiZ7shS1y0kPWr3Jg3EpETySmRaYIsbV2PNeQwgLKtW4BuejSYzfC8ByWSD8txRebZ2adh4Mp~mjh0lsiB1cpkBGBtebBc3w~~Lp8DagwSKP3-YQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Reduced male fertility found in different cPGI-deficient lines. A, A representative inflorescence stem showing the seed setting of the heterozygous cpgi-3(+/−) mutant, the cpgi knockout mutant expressing p35S::cPGI-YFP [p35S::cPGI-YFP cpgi-3(−/−)], the transgenic line overexpressing p35S::cPGI-YFP (cPGI-ox), the cPGI co-suppressed line (cPGI-co), the WT plant (Col-5), and the RNAi knockdown lines Ri-cpgi-1, Ri-cpgi-3, and Ri-cpgi-10. B, Alexander-stained whole-mount anthers were inspected using light microscopy for the viability of mature pollen. A representative image is shown for the plants shown in the same column in (A). Note that most of the pollen degenerated in those plants, producing mostly seedless siliques, while cpgi-3(+/−), cPGI-ox, WT, and Ricpgi-10 plants produced viable pollen and thus produced fertile siliques. Bars in (A) and (B) are 1 cm and 50 µm, respectively.
