Yeast one-hybrid and dual-luciferase reporter assays and EMSAs of type-B LlRRs with the LlWOX9 and LlWOX11 promoters. A, Division of the LlWOX9 and LlWOX11 promoters into fragments according to the location of type-B RR binding elements (GATT/C). B, Y1H assays between five type-B LlRRs and LlWOX9 promoter fragments. C, Y1H assays between type-B LlRRs and LlWOX11 promoter fragments. D, The transient activation test in N. benthamiana leaves verified the transcriptional activation ability of the five type-B LlRRs toward the LlWOX9 and LlWOX11 promoters. Values are means ± sd (n = 3). Asterisks indicate significantly different values (Student’s t test, **P < 0.01). e, The binding ability of His-LlRR1 protein toward the proLlWOX9-1 and proLlWOX11-2 fragments was verified by EMSAs. The binding element GATT was mutated to TTTT in the mutant probe.