Modulations in levels of fruit firmness in SlERF.D7 OE and RNAi fruits. A, Fruit firmness analysis in WT, SlERF.D7 OE, and RNAi line fruits at different stages of ripening, with fruits being harvested at MG stage. A total of 15 fruits were used for each measurement, and the values shown are the means ±sd. B, RT-qPCR analysis of polygalacturonase gene (SlPG2A) and PL (SlPL) at MG, Br, 3-day after Br (Br+3), 7-day after Br (Br+7), and 10-day after Br (Br+10) in SlERF.D7 OE, RNAi, and WT fruits. GAPDH and Actin were used as the internal controls. The error bars represent ±sd of three biological replicates. Asterisks indicate the statistical significance using Student’s t test: *, 0.01 < P-value < 0.05; **, 0.001 < P-value < 0.01.