PavDof2/6/15 bind to the promoters of structural genes related to fruit softening. A, Y1H analysis of PavDof2/6/15 binding to the PavQRT3, PavPME44, PavPL18, PavXTH31, and PavXTH26 promoters. The vertical lines indicate Dof-binding sites (A/TAAAG), and the rectangles represent the promoter fragments used for Y1H. AD, pGADT7 empty vector; SD, synthetical defined medium; AbA, aureobasidin A. B, EMSA showing the binding of PavDof2/6/15 to the PavQRT3, PavPME44, PavPL18, PavXTH31, and PavXTH26 promoter fragments containing Dof-binding sites. Unlabeled probes were used for competition assays; − and + represent absence or presence, respectively.