Three-dimensional imaging to segment ICs. A, Example of a S. moellendorffii root generated from a whole-mount confocal stack (Supplemental Movie S2). Individual cells are randomly colored. Insets show magnifications of the front view or the 270-degree-clockwise turned view. Scale bar: 20 µm. B, Different views of five ICs segmented from confocal stacks. The fifth, blue IC was segmented from the root in panel A. See Supplemental Movies S3–S7 for a full overview of the ICs. Scale bars: 2 µm. C and G, SBF-SEM longitudinal sections (xz-views) showing two ICs. The inset shows a magnification of the indicated area in (C). The asterisks indicate the ICs. Scale bars: 10 μm. D–F and H–J, Serial section reconstruction of the ICs. The numbers indicate the corresponding faces of the cells. See Supplemental Movies S8 and S9 for a full overview. The solid and dashed lines in (H–J) separate the different faces. Scale bars: 1 μm.