AtPRMT5 interacts with AtLCD. A, Subcellular localizations of the AtLCD and AtPRMT5 proteins. Confocal images of N. benthamiana leaf cells harboring the pMDC43-AtLCD or pMDC43-AtPRMT5 constructs. AHL22 was used as an indicator of nuclei; BF, bright field. Scale bar, 10 μm. B, The interaction between AtLCD and AtPRMT5 in N. benthamiana leaves. Pairwise expression of nYFP-AtPRMT5, cCFP-AtPRMT5, nYFP-AtLCD, cCFP-AtLCD, and cCFP-GUS constructs in N. benthamiana. AHL22 was used as an indicator of nuclei. BF, bright field. cCFP-GUS was used as a negative control. Scale bar, 10 μm. C, The combination of AtLCD and AtPRMT5 in 14-day-old Arabidopsis. Protein extracts of WT were immunoprecipitated by anti-AtLCD antibody, and then, the precipitates were exposed to anti-AtPRMT5 or anti-AtLCD antibodies. D, Pull-down assays of AtLCD and AtPRMT5. Recombinant protein His-TF-AtPRMT5 binds to Ni-NTA agarose matrix, then incubated with protein extracts from WT plants, the precipitates were immunoblotted with anti-AtLCD antibody.