The rim^R>H CORD7 allele changes NMJ morphology and increases the readily-releasable vesicle pool. (A) Summary graphs for the number of Brp puncta per NMJ, NMJ size and the number of boutons per NMJ in rim^rescue/rim^rescue (grey), rim^R>H/rim^rescue (red) and rim^R>H/rim^R>H (dark red; n = 19, 16 and 18 NMJs from seven, seven and six animals, respectively). (B) Summary graphs for AZ area, the number of Brp localizations (locs.) per individual AZ and Brp localization (loc.) density measured by dSTORM rim^rescue/rim^rescue, rim^R>H/rim^rescue and rim^R>H/rim^R>H (n = 1088, 898 and 855 AZs from 17, 17 and 16 NMJs and six, six and six animals, respectively). (C) Example eEPSCs in response to 20 Hz trains (50 pulses) in the three genotypes. Scale bars = 200 ms and 20 nA. (D) Cumulative eEPSC amplitudes in rim^rescue/rim^rescue, rim^R>H/rim^rescue and rim^R>H/rim^R>H (n = 9, 7 and 10 NMJs and animals, respectively). Back-extrapolation of linear fits to the average cumulative eEPSC amplitude of the last 20 pulses (straight lines) yielded estimates for the readily releasable pool of 174, 226 and 248 for the three genotypes. (E) Summary graphs for the estimates of back-extrapolations of the cumulative eEPSC amplitudes in rim^rescue/rim^rescue, rim^R>H/rim^rescue and rim^R>H/rim^R>H. Whisker plots show mean ± SEM, scatter plots individual data-points. All displayed measurements were performed with L3 larvae carrying indicated rim alleles (rim^x/rim^x). See also Supplementary Tables 6 and 7.