Cell death in gsl1 gsl10 double mutant under low-Ca conditions. (A) Trypan blue staining of the first or second true leaves of Col-0, gsl1-5, gsl10-5 and gsl1 gsl10 double mutant seedlings. Plants were grown under 2 mM Ca for 5 d, then transferred to 2 mM or 0.2 mM Ca for 2 d. Scale bar = 500 µm. (B) Percentage of lesion area per leaf area. In total, 11–22 individual first and second true leaves were used for quantification. Different letters indicate significant differences under each Ca condition (Steel–Dwass test, P < 0.05). ns = no significant difference.