Zinc-deficient atg41Δ mutants accumulate more total sulfur than wild-type (WT) cells. (A) WT cells (filled squares) and atg41Δ mutants (open squares) were grown to midlog phase in zinc-replete medium (T = 0) and then transferred to zinc-deficient medium. Cells were sampled at 4-hr intervals for analysis by inductively coupled plasma atomic emission spectrometry. Data for zinc, sulfur, potassium, phosphorus, and magnesium are plotted normalized to dry mass of the cell pellet. (B) WT and atg41Δ mutant cells were grown in zinc-deficient, low-sulfur limited zinc medium supplemented with 40 mM (NH₄)₂SO₄, 670 µM methionine, or both. For all panels, cultures were assayed in triplicate and error bars represent 1 SD. The asterisks denote P < 0.01 by Students t-test.