Expression pattern analysis of OsCEN2. A, Transcript expression levels of OsCEN2 in rice seeds on different days after pollination. Mean ± SD; n = 3 biological replicates. B, Subcellular localization of the GFP-OsCEN2 fusion protein in rice protoplasts. D53-mCherry was used as the nuclear marker. The free GFP was used as a control. Scale bars: 20 µm.