Suppression of the phenotype of cbl2 cbl3 mutant by additional mutations in MGT1 and MGT2. A, RT-PCR analysis of MGT1, MGT2 as well as CBL2 and CBL3 gene expression in wild-type (Col-0), double mutant (mgt1 mgt2, cbl2 cbl3), and quadruple mutant (mgt1/2/cbl2/3) plants. ACTIN2 was analyzed as an internal control. B, Growth phenotype of 4-week-old plants of different genotypes in the soil. Scale bar = 5 cm. C and D, Rosette diameter (C) and shoot biomass (D) of different genotypes as shown in (B). Data represent means ± SD (n = 12). Statistical analyses between different genotypes were performed by one-way ANOVA followed by a Turkey’s multiple comparison test. Different letters indicate significant difference at P < 0.05. E, Growth phenotype of 2-week-old young seedlings of different genotypes on the medium containing 0.25 mM or 4 mM Mg²⁺. F and G, Seedling fresh weight (F) and leaf chlorophyll (G) of different genotypes as shown in (E). Data represent means ± SE (n = 4). Columns with different letters indicate statistically significant differences performed by one-way ANOVA followed by a Turkey’s multiple comparison test (P < 0.05).