Simultaneous measurement of net CO₂ assimilation, g_s, and intercellular CO₂ in WT and T27 leaves. A, Net CO₂ assimilation (A_net), (B) g_s, and (C) intercellular CO₂ (C_i) from 30 min of dark into 90 min of light. A_net, g_s, Ci values are the means ± se, n = 7, WT; n = 8, T27; differences in A_net between WT and T27 for measurements at 45–60 min are statistically significant (P < 0.05, two-tailed Student’s t test). Differences in g_s between WT and T27 for measurements at 48–60 min are statistically significant (P < 0.05, two-tailed Student’s t test).