Fig. 4
Effect of daylength on the floral induction of cabbage scions grafted onto G2 × CH F1 radish rootstocks. Germinated seeds of G2 × CH F1 were vernalized at 2°C for 7 d before sowing. (A) Light conditions during the grafting experiment. LD condition (16 h/8 h, light/dark); MD condition (12 h/12 h, light/dark). Grafting was performed ∼20 DAS in LD/LD and LD/MD treatments and ∼23 DAS in MD/LD treatment when the stem of rootstocks reached 7 cm in length. (B) Days to bolting from sowing of rootstocks. (C) Total area of leaves left on rootstocks at the day of grafting. (D) The maximum stem diameter of the grafted scion at 35 DAG. (E) The RsFT transcription level in the leaf of rootstocks at 35 DAG. RsActin was used as an internal control. (F) Floral status of the grafted scions at 63 DAG. (G) Accumulation of FT protein in the grafted cabbage scion at 35 DAG. Different letters indicate statistical difference between the light conditions with the Tukey–Kramer test (P < 0.05, n = 6) in (B), (C) and (D) and with pairwise Wilcoxon tests with the Bonferroni adjustment for multiple comparisons (P < 0.05, n = 6) in (E) and (G).

Effect of daylength on the floral induction of cabbage scions grafted onto G2 × CH F1 radish rootstocks. Germinated seeds of G2 × CH F1 were vernalized at 2°C for 7 d before sowing. (A) Light conditions during the grafting experiment. LD condition (16 h/8 h, light/dark); MD condition (12 h/12 h, light/dark). Grafting was performed ∼20 DAS in LD/LD and LD/MD treatments and ∼23 DAS in MD/LD treatment when the stem of rootstocks reached 7 cm in length. (B) Days to bolting from sowing of rootstocks. (C) Total area of leaves left on rootstocks at the day of grafting. (D) The maximum stem diameter of the grafted scion at 35 DAG. (E) The RsFT transcription level in the leaf of rootstocks at 35 DAG. RsActin was used as an internal control. (F) Floral status of the grafted scions at 63 DAG. (G) Accumulation of FT protein in the grafted cabbage scion at 35 DAG. Different letters indicate statistical difference between the light conditions with the Tukey–Kramer test (P < 0.05, n = 6) in (B), (C) and (D) and with pairwise Wilcoxon tests with the Bonferroni adjustment for multiple comparisons (P < 0.05, n = 6) in (E) and (G).

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