Ngal1 growth in hydroponic culture. (A) Shoot phenotype of wild-type (Col-0) and ngal1 mutant alleles grown in an open hydroponic culture for 30 d under low-B (−B) and control-B (+B) conditions. The inhibited young rosette leaf is shown in the box with an arrow. Inhibition of rosette leaf growth in plants was determined by counting the plants that had the inhibited leaves from the indicated n-number of plants and represented as a percentage (A, bottom part). Scale bar: 1 cm. (B) and (C) Rosette leaf FW was determined in plants grown under low-B and control-B conditions, respectively. Data are mean of n = 45–50 measurements. Letters indicate no significant differences (Dunnett’s test). B concentrations in the treated conditions were as follows: low B (−B: 0.1 µM B) and control B (+B: 30 µM B).