ABA signaling is involved in ethanol-mediated drought tolerance. The roots of 19-day-old WT plants were treated with 10 mM ethanol for 3 d and then subjected to drought stress. (A) Leaf temperature at 72 h after the ethanol treatment (0 DAD). (B) 1 DAD. (C) 3 DAD. (D) Stomatal aperture under 7-d watered control conditions and at 7 DAD. Asterisks indicate significant differences (compared with the control conditions) according to the Student-Newman-Keuls (SNK) test (P < 0.05); (A–C) n > 20 and (D) n = 4. (E, F) Drought test of water- and ethanol-treated abi1-1 plants. (G) Stomatal aperture of WT (Ler) and the abi1-1 mutant at 1-day after 3-day ethanol treatment. (H) Leaf temperature measurement of WT (Ler) and the abi1-1 mutant at 3-d ethanol treatment. There were no significant differences between the control and ethanol-treated abi1-1 plants according to the SNK test (P < 0.05); n = 3.