Synthetic genetic interactions of Yhc1 C-terminal truncations. (Top panel) Plasmid shuffle assays were used to test whether the truncated YHC1 alleles were functional in the genetic backgrounds specified in the left column. Synthetic lethality was indicated by failure to form colonies on FOA agar after 8 d at 20, 30 and 37°C. Cultures of viable FOA-resistant cells were grown in YPD broth at 30°C and the growth phenotypes were assessed by spotting serial 10-fold dilutions. Exemplary spot tests are shown in the bottom panel. Growth is tabulated in the top panel as follows: +++ denotes growth similar to the pertinent strains bearing wild-type YHC1; ++ indicates smaller colony size than the strain bearing wild-type YHC1; ts signifies a temperature-sensitive growth defect; cs indicates a cold-sensitive growth defect.