HLA class I expression profiles on primary endometrial stromal cells based on flow cytometry analysis. (A) The expression of the HLA class I molecules is shown in a CD45⁻CD90⁺ population. The plots depict Mean Fluorescence Intensity (MFI) of HLA-ABC (HLA class Ia), HLA-F and HLA-G. The data are shown as mean±SD (paired t-test). Below, histograms of HLA-ABC, HLA-F and HLA-G staining within the CD45⁻CD90⁺ selection (pink). (B) Western blot of HLA-F (42 kDa) and HLA-G (45 kDa) on endometrial stromal cells (ESC) and on the choriocarcinoma cell line JEG3 (JEG3). α-Actinin (110 kDa) serves as loading control.