Impulse behaviour of AABAA. We show the number of simulations (out of 400) have only the central binding site bound, over time, for the AABAA configuration compared with (A) AA-B-AA and ABCDE and (B) ABA. For example, in the AABAA scenario, the y-axis represents the number of simulations out of the 400 in which the B site is bound and none of the A sites are bound. Note that we start the simulation with ‘naked’ DNA (no TFs bound).