Integration pattern near LMO2 in K562 and HepG2 cells. (A) Integrations in K562 cells. Bars indicate the sum of unique integration events color-coded to match the genomic state in which they integrated (left axis); there are 471 events represented in this ∼110-kb span. Bar tops are highlighted with a black line and a dot for visibility. The right axis indicates the rate of integration in a 1-kb sliding window for both experimental integrations (blue) and a representative in silico random control (gray). The location of LMO2 is represented in the lower right of the figure. Approximately 0.15% of the 315 810 integrations scored landed in this LMO2 interval, which comprises 0.0036% of the genome. The colored track below the integrations is the chromatin state segmentation track (21); colors correspond to different states, as indicated. (B) Integrations in HepG2 cells. The scales are the same as in (A). There are 19 integrations in this region. Experimental integrations are significantly depleted relative to random control integrations in this region (bootstrapping; P < 0.0001). Note that the number of random integrations per kilobase tends to be higher for HepG2 than K562 cells. This is because there are >10-fold as many integrations in HepG2 relative to K562, resulting in a corresponding increase in the number of random control integrations. This figure clearly shows the cell-type-specific differences in the raw number of experimental integrations in the LMO2 region between K562 and HepG2 cells. These differences can likely be attributed to the presence of active LMO2 enhancers and promoters in K562 cells and their absence in HepG2 cells.