Fig. 4.
Fancd2 Lysine 595 is required for Mre11 foci removal during mitosis. a) Mre11 and gH2Av recruitment to fancd2K595R papillar cells +/− hs-I-CreI. DNA (DAPI), magenta; Mre11, green; gH2Av, red. Yellow arrows = Mre11 + gH2Av + foci. Hatched box = area magnified 10× in the corresponding inset below each panel. White arrowhead = micronucleus. Hatched outline = nuclei. Scale bars = 10 μm. NEBD = Nuclear Envelope Break Down. b) Quantification of Mre11+ gH2Av+ foci recruitment in fancd2K595R papillar cells +hs-I-CreI pre- and post- the first pupal mitotic division of the pupal stage (first mitotic stage). Focus index= average number of foci/cell in papillar cells of a given animal. Each data point represents a single animal (N). Statistical test: Ordinary 1-way ANOVA. See Materials and methods for statistical notations.

Fancd2 Lysine 595 is required for Mre11 foci removal during mitosis. a) Mre11 and gH2Av recruitment to fancd2K595R papillar cells +/− hs-I-CreI. DNA (DAPI), magenta; Mre11, green; gH2Av, red. Yellow arrows = Mre11 + gH2Av + foci. Hatched box = area magnified 10× in the corresponding inset below each panel. White arrowhead = micronucleus. Hatched outline = nuclei. Scale bars = 10 μm. NEBD = Nuclear Envelope Break Down. b) Quantification of Mre11+ gH2Av+ foci recruitment in fancd2K595R papillar cells +hs-I-CreI pre- and post- the first pupal mitotic division of the pupal stage (first mitotic stage). Focus index= average number of foci/cell in papillar cells of a given animal. Each data point represents a single animal (N). Statistical test: Ordinary 1-way ANOVA. See Materials and methods for statistical notations.

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