AAV9SLR-mediated generation of AP-1 cons hp dONs in aortic grafts of mgR/mgR mice significantly decreases monocyte infiltration in the aortic wall. (A) MCP-1 mRNA level was assessed in transduced tissue and compared to the age-matched wild type values as control. RPL32 was used as a housekeeping gene (n = 6, ***P < 0.001). (B) Representative confocal images showing F4/80 (red) immunohistochemistry demonstrating macrophage presence in the aortic sections of the indicated treatment groups. Elastin autofluorescence was recorded in the green channel and DAPI (blue) was used to counterstain nuclei. (C) Statistical quantification of macrophage density in the adventitia of aortic cryosections from mgR/mgR mice (n = 6 for mgR/mgR mice, 20 images analysed/group, **P < 0.01). The scale bar represents 50 μm. Data were analysed using one-way analysis of variance (ANOVA), followed by a post hoc Tukey multiple comparison test.