Performance of MutateX predictions when ran on p53 DNA-binding domain molecular dynamics ensembles. The plots illustrate the correlation between available experimental ΔΔG values and MutateX predictions. We performed MutateX scans on the original X-ray experimental structure, the starting structure of an MD simulation and MD simulation ensembles of 25, 50 and 100 frames extracted from the same MD trajectory. For the ensemble runs, we show the average ΔΔG values calculated over independent FoldX runs performed on each ensemble structure. The horizontal lines show the SD of experimental measurements when multiple values were found for any given mutation, while vertical lines account for the SD of the predicted values on the ensemble.