Validation of RNA-seq data for the representative target genes by RT-qPCR. A, RNA-seq data of eight representative DEGs in BZR1-OX2 seeds at 2 HAI. The gene expression levels in Nip were set to 1. B, Verification of the DEGs by RT-qPCR in both BZR1-OX2 and bzr1-i1 mutant seeds at 2 HAI. The gene expression levels in Nip were set to 1. Actin1 was used as the internal control for normalization of gene expression. Error bars represent sd (n = 3 experiments, each replicate contained 30 seeds). *P < 0.05; **P < 0.01 (Student’s t test).