The BZR1-RAmy3D transcriptional module also plays essential roles in tissues of the embryo. A–F, Microscopic observation of starch granules that have accumulated in the shoots of Taichung 65 (TC65) (A–C) and d61 (D–F) at 36 HAI. The samples were embedded in LR white resin, cut into semi-thin sections, and stained with iodine solution. (B) and (C) are enlargements of the rectangles marked with blue boxes on the inside parts and green boxes on the outside parts of the shoots in (A), respectively. E and F are enlargements of the rectangles marked with blue boxes on the inside parts and green boxes on the outside parts of the shoots in (D), respectively. Scale bar = 20 µm. G, Statistical analysis of the average number of starch granules per cell in shoots of the d61 mutant and TC65 control. H–M, Microscopic observation of the starch granules that have accumulated in the shoots of Nip (H–J) and the bzr1-i1 mutant (K–M) at 36 HAI. I and J are the enlargements of the rectangles marked with blue boxes on the inside parts and green boxes in the outside parts of the shoots in (H), respectively. L and M are the enlargements of the rectangles marked with blue boxes on the inside parts and green boxes on the outside parts of the shoots in (K), respectively. N, Statistical analysis of the average number of starch granules per cell in shoots of the bzr1-i1 mutant and Nip control. In (G) and (N), error bars represent sd (n = 3 experiments, each replicate contained 30 cells). Different letters indicate significant differences at P < 0.05 by Duncan’s multiple range test. O and P, Morphology (O) and quantitative analysis (P) of the plumule lengths in the d61 mutant and TC65 control cultivated in vitro for 120 h supplemented with Glc or Man or the mock control. Q and R, Morphology (Q) and quantitative analysis (R) of the plumules length in the bzr1-i1 mutant and Nip control cultivated in vitro for 120 h supplemented with Glc or Man or mock. Glc, glucose; Man, mannitol. Scale bar = 1 mm. Error bars represent sd (n = 3 experiments, each replicate contained 20 plumules). **P < 0.01 (Student’s t test). The images were digitally extracted for comparison in (O) and (Q).